Injectable Biopolymer-hydroxyapatite Hydrogels: Obtaining and their Characterization

Authors L.B. Sukhodub1 , G.O. Yanovska2 , V.M. Kuznetsov2 , O.O. Martynyuk2, L.F. Sukhodub2

1 SI "Institute of Microbiology and Immunology, the Mechnikov I.I. AMS of Ukraine", 14/16, Pushkinska St., 61057 Kharkiv, Ukraine

2 Sumy State University, 2, Rymskogo-Korsakova St., 40007 Sumy, Ukraine

Issue Volume 8, Year 2016, Number 1
Dates Received 21 December 2015; published online 15 March 2016
Citation L.B. Sukhodub, G.O. Yanovska, V.M. Kuznetsov, et al., J. Nano- Electron. Phys. 8 No 1, 01032 (2016)
DOI 10.21272/jnep.8(1).01032
PACS Number(s) 87.85.J –, 87.64.Bx, 87.64.Ee
Keywords Hydroxyapatite, Chitosan, Sodium alginate, Hydrogels.
Annotation Hydrogels based on hydroxyapatite (HA) and Chitosan (CS) with addition of sodium alginate (Alg) were synthesized by in situ precipitation method. Structure, morphology, chemical and phase composition of the HA/CS and HA/CS/Alg hydrogels were characterized by TEM, FTIR and XRD. Hydrogels consist of low crystallinity calcium deficient hydroxyapatite (JCPDS 9 432), the needle-like crystallites have an average size 25 nm. The introduction of Alginate powder into HA/CS hydrogel solution demonstrate the viscosity enhancing of the HA/CS hydrogel due to polyelectrolyte reaction between Alginate and Chitosan macromolecules. Two natural polymers and partially released from hydroxyapatite Ca2+ ions formed a matrix by crosslinking the polymer macromolecules through hydroxyl, amino and carbonyl groups. These processes promote the formation of a more stable structure of HA/CS/Alg hydrogel as compared to HA/CS. The structural integrity and degradation tests have demonstrated that HA/CS/Alg1.0 saved its initial shape in 7 days of shaking in SBF solution, meanwhile for HA/CS, a structural decay was observed. The HA/CS hydrogel had completely lost its volume support after 1 day shaking in SBF. Thus, the ability of HA/CS hydrogel to maintain its shape with implantation into bone tissue defect may be enhanced with alginate addition, but alginate content more than 1 w/w % reduces the hydrogel plasticity, increases the swelling and accelerates the shape decay.