Calculation of Intracellular Pressure of Red Blood Cells at Jaundice According to Atomic Force Microscopy Data

Authors Yu.S. Nagornov1 , I.V. Zhilyaev2

1 Togliatti State University, 14b, Belorusskaya St., 445667 Togliatti, Samara region, Russia

2 Southern Federal University, 105/42, Bolshaya Sadovaya St., 344006 Rostov-on-Don, Russia

Issue Volume 8, Year 2016, Number 1
Dates Received 29 November 2015; published online 15 March 2016
Citation Yu.S. Nagornov, I.V. Zhilyaev, J. Nano- Electron. Phys. 8 No 1, 01030 (2016)
DOI 10.21272/jnep.8(1).01030
PACS Number(s) 64.75.g, 87.17.Aa
Keywords Atomic force microscopy (9) , Erythrocyte membrane biomechanics, Intracellular pressure, Obstructive jaundice.
Annotation The present work is devoted to the analysis of three-dimensional data of atomic force microscopy for research of the morphology of red blood cells. In this paper we built a biomechanical model of the erythrocyte, which allowed calculating the intracellular pressure of erythrocyte based on data of atomic force microscopy. As a result, we obtained the dependence intracellular pressure on the morphology of red blood cell. We have proposed a method of estimating of intracellular pressure of erythrocytes based on numerical modeling and data of atomic force microscopy of erythrocytes scan, which involves a comparison of the experimental data with the results of numerical calculation. The method is applied to the data of atomic force microscopy of erythrocytes of experimental animals - dwarf domestic pigs with different degrees of obstructive jaundice and normal. It is shown that with increasing severity of the disease and the concentration of bilirubin in the blood there is an infringement erythrocyte membranes, by an average increasing their volume and intracellular pressure.