Extracellular Synthesis of Zinc Oxide Nanoparticles Using Thermo-Halotolerant Aeribacillus pallidus strain SJP 27: Characterization and Antibacterial Potential

Authors Jagdish Parihar, Ashok Kumawat , Kamakhya Prakash Misra , Ashima Bagaria

Department of Physics, Manipal University Jaipur, Jaipur-303007, Rajasthan, India

Е-mail ashima.bagaria@jaipur.manipal.edu
Issue Volume 13, Year 2021, Number 2
Dates Received 10 January 2021; revised manuscript received 23 March 2021; published online 09 April 2021
Citation Jagdish Parihar, Ashok Kumawat, Kamakhya Prakash Misra, Ashima Bagaria, J. Nano- Electron. Phys. 13 No 2, 02007 (2021)
DOI https://doi.org/10.21272/jnep.13(2).02007
PACS Number(s) 78.67.Bf, 33.20.Ea
Keywords Aeribacillus pallidus, Thermo halotolerant, Zinc oxide nanoparticles, FTIR (26) , Energy band gap and extracel-lular synthesis.

The current work reports the extracellular synthesis of zinc oxide (ZnO) nanoparticles (NPs) using the bacterial isolate Aeribacillus pallidus strain SJP 27 (Accession No. MW148443) from soil sample of arid and semi-arid regions of the Great Indian Thar desert. Bacterial cells were grown overnight at 60 °C incorporating a halo-tolerance of 5 % w/v NaCl. Physiochemical characterization of ZnO NPs were carried out using UV-Visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The antimicrobial activity of synthesized ZnO NPs was confirmed by minimum inhibitory concentration (MIC) against Escherichia coli (8 mg/ml) and Staphylococcus aureus (4 mg/ml). The present study encourages the use of bacterial isolates for the extracellular synthesis of ZnO NPs. To the best of our knowledge, this is the first ever reported study of a thermo-halotolerant, Aeribacillus pallidus for extracellular synthesis of ZnO NPs in particular.

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