Calculation of Intracellular Pressure of Red Blood Cells at Jaundice According to Atomic Force Microscopy Data

Автор(ы) Yu.S. Nagornov1 , I.V. Zhilyaev2
Принадлежность

1 Togliatti State University, 14b, Belorusskaya St., 445667 Togliatti, Samara region, Russia

2 Southern Federal University, 105/42, Bolshaya Sadovaya St., 344006 Rostov-on-Don, Russia

Е-mail nagornov.yuri@gmail.com
Выпуск Том 8, Год 2016, Номер 1
Даты Получено 29.11.2015, опубликовано online – 15.03.2016
Ссылка Yu.S. Nagornov, I.V. Zhilyaev, J. Nano- Electron. Phys. 8 No 1, 01030 (2016)
DOI 10.21272/jnep.8(1).01030
PACS Number(s) 64.75.g, 87.17.Aa
Ключевые слова Atomic force microscopy (9) , Erythrocyte membrane biomechanics, Intracellular pressure, Obstructive jaundice.
Аннотация The present work is devoted to the analysis of three-dimensional data of atomic force microscopy for research of the morphology of red blood cells. In this paper we built a biomechanical model of the erythrocyte, which allowed calculating the intracellular pressure of erythrocyte based on data of atomic force microscopy. As a result, we obtained the dependence intracellular pressure on the morphology of red blood cell. We have proposed a method of estimating of intracellular pressure of erythrocytes based on numerical modeling and data of atomic force microscopy of erythrocytes scan, which involves a comparison of the experimental data with the results of numerical calculation. The method is applied to the data of atomic force microscopy of erythrocytes of experimental animals - dwarf domestic pigs with different degrees of obstructive jaundice and normal. It is shown that with increasing severity of the disease and the concentration of bilirubin in the blood there is an infringement erythrocyte membranes, by an average increasing their volume and intracellular pressure.

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